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assess proteins photo-stability of cetuximab by Circular dichroism (CD) (CAT#: STEM-MB-0622-WXH)
Introduction
Cetuximab is a type of targeted cancer drug. It is also known as Erbitux. It is a treatment for bowel cancer that has spread (advanced bowel cancer) and head and neck cancers. Depending on your cancer type, you might have cetuximab alongside other chemotherapy drugs or radiotherapy treatment.
Principle
Circular dichroism (CD) is a spectroscopy technique that measures the absorption difference between left and right circularly polarized light. By symmetry, this asymmetric absorption can only occur for asymmetric molecules, meaning chiral molecules.
Applications
Circular dichroism (CD) spectroscopy is a powerful technique that is sensitive to the chirality (handedness) of molecules. It can be used to study absolute stereochemistry, enantiomeric composition, racemization, enantiomeric differentiation, and molecular interactions and conformation.
Procedure
1. Sample preparation
2. Measurement by CD instrument
3. Data analysis
2. Measurement by CD instrument
3. Data analysis
Materials
Circular dichroism (CD) spectrophotometer
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