Antibody Humanization (CAT#: STEM-MB-0131-WXH)

Introduction

Antibody humanization is an important part of experimental research on the production and preparation of recombinant antibodies (monoclonal antibodies). The so-called antibody humanization is the process of developing from murine antibody to human antibody. There are various restrictions on the use of mouse-derived antibodies. Although mouse antibodies are specific to the target antigen and can specifically bind to the target antigen, they cannot activate the corresponding human effector system, so that the normal antigen-antibody reaction cannot occur; in addition, When mouse antibodies enter the human body as foreign proteins, the human immune system will respond and produce specific antibodies with mouse antibodies as antigens, that is, human anti-mouse antibodies (HAMA). It is cleared quickly from the body and has a short half-life. Due to various limitations in the clinical application of mouse-derived antibodies, people use recombinant DNA technology to humanize mouse-derived antibodies to humanize antibodies.<br />The humanization of murine antibodies is to make them have a very similar profile to the antibody molecules in the human body through genetic modification, so as to evade the recognition of the human immune system and avoid the induction of HAMA reactions.