Anti-Human CD3 mAb, Seafrom (CAT#: STEM-CT-0001-ZJF)

The Anti-Human CD3 mAb manufactured by Seafrom is suitable for the stimulation and modulation of T cell responses in cell therapy.
In immunology, the co-receptor for CD3 (Cluster of Differentiation 3) T cells is a protein complex. CD3 consists of five peptide chains, namely γ chain, δ chain, ε chain, ζ chain, and η chain, all of which are transmembrane proteins. The transmembrane region of CD3 molecules is connected to the transmembrane regions of the two peptide chains of the T-cell receptor (TCR) through salt bridges, forming the TCR-CD3 complex, which collectively participates in the recognition of antigens by T cells. The activation signals generated by TCR recognition of antigens are transduced into T cells by CD3.
Individual nucleated cells isolated from peripheral blood, bone marrow, or cord blood, when cultured for a certain period in the presence of CD3 monoclonal antibodies and various cytokines, can yield a substantial proliferation of T lymphocytes.
Soluble method: It is recommended to use a concentration of 500ng/mL, under which conditions, cells can achieve sufficient activation and stimulation, leading to a significant increase in cell proliferation and the final CD3+CD56+ double-positive rate.
Coating method: Coat a T75 flask with 10mL of PBS buffer (5μg/mL anti-human CD3) at 4°C overnight. Before use, remove the PBS buffer and wash three times with physiological saline.
Storage: Store at 2~8°C with a shelf life of 24 months. If needed for aliquoting, aliquots can be stored at -20°C for 6 months or at -80°C for 12 months, avoiding repeated freeze-thaw cycles.

Expression Host: CHO cells
Purity: >95%, analyzed by SDS-PAGE gel and HPLC
Endotoxin Level: <0.01 EU/µg
Activity: Binding rate with Jurkat cells not less than 90%
Purification Method: Protein A affinity chromatography
Appearance: Colorless transparent liquid

High purity, activity, and stability
High batch to batch consistency
Gmp production management standards

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