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Analysis of Visfatin (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0629-LGZ)
Introduction
Visfatin, also known as nicotinamide phosphate nucleoside transferase (NAmPRT enzyme or NAMPT), formerly known as pre-B cell colony enhancer factor 1 (PBEF1), is an enzyme encoded by the NAMPT gene. Visfatin is abundant in visceral fat in both humans and mice, and its plasma levels increase during the development of obesity.
Principle
MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.
Applications
Metabolic, Immunology/Inflammation
Procedure
1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.
Materials
• Sample Type: Plasma, Serum
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