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Analysis of VEGF-C (Human) by ELISA (CAT#: STEM-MB-1259-LGZ)

Introduction

Vascular endothelial growth factor (VEGF) is a key factor in the formation of new blood vessels. VEGF can induce the regeneration of existing blood vessels or the growth of new blood vessels, so it is the key to embryonic development and blood vessel repair. VEGF can also be used by solid tumors to promote their growth. The VEGF family includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E and PIGF-1, PIGF-2.




Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Cardiovascular

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or other biological fluids