Analysis of uPAR/PLAUR/CD87 by ELISA (CAT#: STEM-MB-1586-LGZ)

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

uPAR is part of the plasminogen activation system, which in healthy organisms is involved in tissue reorganization such as mammary gland regression and wound healing. uPAR is involved in other cancer-related processes such as cell migration, cell cycle regulation, and cell adhesion.

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

• Sample Type: Serum, plasma, cell culture supernatant and other biological samples