Analysis of tumor marker by surface enhanced laser desorption/ionization (SELDI) (CAT#: STEM-ST-0335-LJX)

Introduction

Proteomic is the study of proteomes and it involves the global analysis of protein expression profiles, the identification of them and their function in any tissue, organ or organism. Oncoproteomic is the application of proteomics technologies in oncology, which promises to accelerate the discovery of new therapeutics targets and useful biomarkers for diagnosis, prognostic and medical treatment of diverse diseases including cancer. In this context, SELDI (surface-enhanced laser desorption-ionization) is one of the more used techniques for the development of new tools with clinical utility, among them the discovery of new biomarker molecules that could serve as indicators of both physiological and disease states. This review covers general aspects of the proteomic techniques and particularly we describe the findings of some studies that used SELDI for the generation of proteomic patterns in order to identify potential biomarkers associated with the diagnosis and prognosis of a variety of cancers.

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Principle Applications Procedure Materials Notes Related Products

Principle

The surface enhanced laser desorption ionization technique belongs to laser desorption mass spectrometry (LDMS). It is different from ordinary LDMS in that the laser is not directly hit on the sample to desorption, but the sample is suspended in the matrix, the laser is hit on the matrix, the matrix absorbs and transmits the laser energy, so that the sample in the matrix desorption out. After desorption and ionization, the samples were examined in a time-flight mass spectrometer.

Applications

For protein analysis and measurement of molecular weight of complete proteins
For the diagnosis of a variety of diseases, especially cancer

Procedure

1. The surface of the protein chip is treated in a certain chemical or biochemical way (surface enhancement), so that it has the ability to bind specifically to a certain type of protein
2. The serum or protein extract is directly added to the surface of the chip, and the chip is washed after incubation. Specific proteins bind to the chip and are thus separated from the protein mixture
3. The chip then uses a "chip reader" (a kind of SELDI-TOF-MS) to obtain a mass spectrum of the protein bound to the chip
4. The SELDI protein chip system can be used to compare changes in the protein profile of any set of control samples or different disease states to identify biomarkers or disease-related targets

Materials

• Sample Type:
Tumor marker

Notes

When operating, strictly follow the experimental steps.

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