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Analysis of TSP-1 (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0614-LGZ)

Introduction

The adhesive glycoprotein thrombocytophenin-1 (THBS1, TSP1) is localized in the extracellular matrix (ECM) and mediates interactions between cells and ECM as well as between cells. Thrombospondin-1 is a multi-domain glycosylated protein that interacts with a variety of extracellular components, including matrix metalloproteinases (MMPs), collagen, cell receptors, growth factors and cytokines.

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Principle Applications Procedure Materials

Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Oncology & Cancer, Toxicology

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum
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