Analysis of Trypsin (TPO) by Raman Spectroscopy (CAT#: STEM-ST-0015-WXH)

Name: Analysis of Trypsin (TPO) by Raman Spectroscopy
SKU: STEM-ST-0015-WXH
Rating: 5 (1 reviews)

Introduction

Trypsin (TPO) is produced by the exocrine cells of the mammalian pancreas. It is secreted as a proenzyme – trypsinogen, which is a part of the pancreatic juice. Trypsinogen (TGN) is converted to TPO by enterokinase, which is produced by the duodenal mucosal cells, and next TPO activates the other proteolytic enzymes. TGN activation within the pancreas is prevented by the presence of inhibitors that are also secreted by the acinar cells.With the autocatalytic ability of TPO there quickly occurs a full activation of separated TGN.

Buy Now

Principle Applications Procedure Materials

Principle

Raman Spectroscopy is a non-destructive chemical analysis technique which provides detailed information about chemical structure, phase and polymorphy, crystallinity and molecular interactions.
The principle behind Raman spectroscopy is that the monochromatic radiation is passed through the sample such that the radiation may get reflected, absorbed, or scattered. The scattered photons have a different frequency from the incident photon as the vibration and rotational property vary.

Applications

• Analysis of biocompatibility of a material.
• Analysis of nucleic acids.
• Study of interactions between drugs and cells.
• Photodynamic therapy (PDT).
• Analyzing metabolic accumulations of a substance or compounds.
• Diagnosis of disease.
• Analysis of individual cells.
• Cell sorting applications.
• Analyzing the features of biomolecules.
• Study of bone structure.