Analysis of Troponin T (cardiac) (Rat) by ELISA (CAT#: STEM-MB-1248-LGZ)

Name: Analysis of Troponin T (cardiac) (Rat) by ELISA
SKU: STEM-MB-1248-LGZ
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Introduction

Troponin (Tn) is a regulatory protein of muscle tissue contraction, located in the fine myofilaments of contractile protein, and plays an important regulatory role in the process of muscle contraction and relaxation. It contains three subtypes: fast -, slow - and cardiac troponin (cTn). While the first two are associated with skeletal muscle, cardiac troponin is found only in cardiac cells and is a complex composed of three subunits, troponin T (cTnT), troponin I (cTnI), and troponin C (cTnC). cTnT and cTnI are antigens specific to cardiomyocytes. cTnI, with a molecular weight of 23.88 KDa, is degraded from myocardial fibers when cardiomyocytes are damaged. Elevated serum cTn reflects myocardial cell damage, and its specificity and sensitivity are higher than those of the commonly used myocardial enzyme profiles.

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Principle Applications Procedure Materials

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Cardiac Biomarkers, Cardiac Injury, Toxicology, Cardiovascular

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.