Analysis of Total Cholesterol and Cholesteryl Ester by Fluorescence Microplate Reader (CAT#: STEM-MB-1504-LGZ)

Total Cholesterol (TC) includes Free cholesterol (FC) and cholesteryl esters (CE). Cholesterol ester can be hydrolyzed into cholesterol and free fatty acids by cholesterol esterase (CE), and cholesterol was oxidized in cholesterol oxidase (CO) to produce 4-cholestenone and hydrogen peroxide. The hydrogen peroxide reacts in the presence of enzyme and probe, and the fluorescence intensity of the produced fluorescent substance at the excitation wavelength of 535 nm and the emission wavelength of 590 nm is proportional to the cholesterol concentration.

Used to detect the contents of total cholesterol and cholesterol ester in serum (plasma), animal tissue and cell samples.

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Determination of fluorescence intensity.
4. Make the mark curve and calculate the result.

• Sample Type: serum, plasma, urine