Analysis of Thermodynamic properties of protein unfolding by Differential Scanning Fluorimetry(DSF) (CAT#: STEM-MB-0756-WXH)

Introduction

Protein folding is the physical process by which a protein chain is translated into its native three-dimensional structure, typically a "folded" conformation, by which the protein becomes biologically functional. Via an expeditious and reproducible process, a polypeptide folds into its characteristic three-dimensional structure from a random coil. Denaturation of proteins is a process of transition from a folded to an unfolded state. It happens in cooking, burns, proteinopathies, and other contexts.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Differential Scanning Fluorimetry measures protein thermal unfolding by monitoring changes in fluorescence emission of a sample upon heating. This allows the determination of protein thermostability and complex formation even with weakly binding ligands by thermal shift assay. Differential Scanning Fluorimetry is therefore ideally suited for screening of optimum buffer conditions like pH, buffer composition and ionic strength. The technique is applicable to any biological sample, from soluble proteins to integral membrane proteins.

Applications

To identify low-molecular-weight ligands that bind and stabilize purified proteins.
To measure the denaturation and unfolding of proteins.

Procedure

1. Preparation of compound solutions
2. Preparation of buffer/additive screen plates
3. Preparation of compound storage plates
4. Equipment preparation
5. Sample preparation
10. Performing the scan

Materials

Real-time PCR instrument

Other recommended products

Detection of Kinase Inhibitor Selectivity by Differential Scanning Fluorimetry(DSF) (CAT#: STEM-MB-0752-WXH)
Analysis of lac operon by Differential Scanning Fluorimetry(DSF) (CAT#: STEM-MB-0812-WXH)
Analysis of Glucose-binding proteins (GBPs) by Differential Scanning Fluorimetry(DSF) (CAT#: STEM-MB-0828-WXH)
Analysis of Nsp3 macrodomain of SARS-CoV-2 by Differential Scanning Fluorimetry(DSF) (CAT#: STEM-MB-0817-WXH)
Monitoring RNA Stability by Differential Scanning Fluorimetry(DSF) (CAT#: STEM-MB-0762-WXH)
Analysis of molecularly imprinted polymer nanoparticles (MIP-NPs) by Differential Scanning Fluorimetry(DSF) (CAT#: STEM-MB-0776-WXH)
Analysis of PMM2 protein by Differential Scanning Fluorimetry(DSF) (CAT#: STEM-MB-0832-WXH)
Analysis of Reverse transcriptase (RT) by Differential Scanning Fluorimetry(DSF) (CAT#: STEM-MB-0835-WXH)