Analysis of TARS2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0306-LGZ)

Name: Analysis of TARS2 Gene (Mutation) by RT-qPCR
SKU: STEM-MT-0306-LGZ
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Introduction

Official Full Name: threonyl-tRNA synthetase 2, mitochondrial
Also known as: thrRS; TARSL1; COXPD21
This gene encodes a member of the class-II aminoacyl-tRNA synthetase family. The encoded protein is a mitochondrial aminoacyl-tRNA synthetase. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 4.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.