Analysis of α-Synuclein (Human) by ELISA (CAT#: STEM-MB-1273-LGZ)

Name: Analysis of α-Synuclein (Human) by ELISA
SKU: STEM-MB-1273-LGZ
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Introduction

α-Synuclein is a neuronal protein that plays multiple roles in synaptic activity. α-Synuclein participates in synaptic vesicle exocytosis as a monomer and regulates synaptic vesicle trafficking and subsequent neurotransmitter release by enhancing vesicle initiation, fusion and expansion of the exocytic fusion pore. Aα-Synuclein plays a role in the regulation of dopamine neurotransmission by binding to the dopamine transporter (DAT1) to regulate its activity, and mutations in its gene are associated with Parkinson's disease.

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Principle Applications Procedure Materials

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Alzheimer’s Disease, Neurobiology/Neurodegeneration

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.