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Analysis of STAT3 (Human, Mouse, Rat) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0552-LGZ)
Introduction
STAT3 is a signal transducer and activator of transcription that mediates cellular responses to interleukins, KITLG/SCF, LEP, and other growth factors. Once activated, coactivators such as NCOA1 or MED1 are recruited to the promoter region of the target gene.
Principle
MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.
Applications
Phosphoproteins, Intracellular Signaling
Procedure
1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.
Materials
• Sample Type: Plasma, Serum
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