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Analysis of SMARCE1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2486-LGZ)

Name: Analysis of SMARCE1 Gene (Mutation) by RT-qPCR
SKU: STEM-MT-2486-LGZ
Rating: 5 (1 reviews)

Introduction

Official Full Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1
Also known as: CSS5; BAF57
The protein encoded by this gene is part of the ATP-dependent large chromatin remodeling complex SWI/SNF, which is required for the transcriptional activation of genes normally repressed by chromatin. Encoded proteins, either alone or in the SWI/SNF complex, can bind to 4-way junction DNA, which is thought to mimic the topology of DNA entering or exiting nucleosomes. The protein contains a DNA-binding HMG domain, but disruption of this domain does not abolish the DNA-binding or nucleosome displacement activities of the SWI/SNF complex. Unlike most SWI/SNF complex proteins, this protein has no yeast counterpart.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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