Analysis of SHLD2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2055-LGZ)

Name: Analysis of SHLD2 Gene (Mutation) by RT-qPCR
SKU: STEM-MT-2055-LGZ
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Introduction

Official Full Name: shieldin complex subunit 2
Also known as: RINN2; FAM35A; FAM35A1; bA163M19.1
Involved in negative regulation of double-strand break repair via homologous recombination; positive regulation of double-strand break repair via nonhomologous end joining; and positive regulation of isotype switching. Located in nucleus and site of double-strand break.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.