Analysis of Reduced Glutathione (GSH) by Ultraviolet-visible Spectrophotometer (CAT#: STEM-MB-1333-LGZ)

Reduced glutathione (GSH) can react with dithio-dinitrobenzoic acid (DTNB) to produce thio-nitrobenzoic acid and glutathione disulfide. Nitromercaptobenzoic acid is a yellow compound. The content of reduced glutathione (GSH) can be determined by colorimetric quantification at 420 nm.

It was used to detect the content of GSH in serum, plasma, animal and plant tissue samples and cultured cells.

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

• Sample Type: serum, plasma, animal and plant tissue samples and cultured cells