Analysis of Proinsulin (Human) by ELISA (CAT#: STEM-MB-1140-LGZ)

Name: Analysis of Proinsulin (Human) by ELISA
SKU: STEM-MB-1140-LGZ
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Introduction

Proinsulin is derived from Proinsulin, which removes its propeptide through proteolytic hydrolysis to produce proinsulin composed of 86 amino acids. Proinsulin enters the Golgi body along with microvesicles in cytoplasm. Under the action of proteolytic enzymes, the three arginine linked chains of 31, 32 and 60 are cut off, and the chain is broken to produce C-peptide without function, and insulin is produced at the same time. It's secreted out of B cells and into the bloodstream.

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Principle Applications Procedure Materials

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Metabolic

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.