Analysis of PRG4 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0589-LGZ)

Name: Analysis of PRG4 Gene (Mutation) by RT-qPCR
SKU: STEM-MT-0589-LGZ
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Introduction

Official Full Name: proteoglycan 4
Also known as: MSF; SZP; CACP; HAPO; JCAP
The protein encoded by this gene is a large proteoglycan synthesized by chondrocytes and some synovial lining cells that line the surface of articular cartilage. This protein contains chondroitin sulfate and keratin sulfate glycosaminoglycans. It acts as a boundary lubricant on the cartilage surface, facilitating the elastic absorption and energy dissipation of synovial fluid. Mutations in this gene result in camptodactyly-arthropathy-coxa vara-pericarditis syndrome. Alternative splicing results in multiple transcript variants.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.