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Analysis of PRAS40 (Human, Mouse, Rat) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0498-LGZ)
Introduction
PRAS40 is a direct substrate of AKT, and it is expressed in various tissues in the human body, especially in the heart and liver. Protein PRAS40 can promote cancer and also has tumor suppressor effect.
Principle
MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.
Applications
Phosphoproteins, Intracellular Signaling
Procedure
1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.
Materials
• Sample Type: Whole Cell Lysate
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