Inquiry Basket

Analysis of PIP5K1A Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0571-LGZ)

Name: Analysis of PIP5K1A Gene (Mutation) by RT-qPCR
SKU: STEM-MT-0571-LGZ
Rating: 5 (1 reviews)

Introduction

Official Full Name: phosphatidylinositol-4-phosphate 5-kinase type 1 alpha
Enables 1-phosphatidylinositol-4-phosphate 5-kinase activity and kinase binding activity. Involved in several processes, including activation of GTPase activity; focal adhesion assembly; and ruffle assembly. Located in several cellular components, including lamellipodium; nuclear speck; and ruffle membrane. Colocalizes with mRNA cleavage and polyadenylation specificity factor complex.

Add to Cart Please Inquire

Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of Lutzomyia Longipalpis by Real-Time PCR Method (CAT#: STEM-MB-3663-LGZ)
Analysis of MAP10 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0208-LGZ)
Analysis of LITAF Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2475-LGZ)
Analysis of EIF3F Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2217-LGZ)
Analysis of KIF5A Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2479-LGZ)
Analysis of RGS8 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0621-LGZ)
Analysis of PTCH2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0101-LGZ)
Analysis of PRG4 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0589-LGZ)

We use cookies to understand how you use our site and to improve the overall user experience. This includes personalizing content and advertising. Read our Privacy Policy

Accept Cookies