High-quality products
Reliable performance
Analysis of Particle Identification by Raman Microscopy (CAT#: STEM-B-0367-CJ)
Introduction
Determining the origin and identity of particulate matter in (bio)pharmaceutical drug products is crucial for troubleshooting and root-cause analysis and, thus, often very time-critical. The identification approach includes the visual inspection of the sample inside the primary container, a high-resolution photo documentation of the particles and a subsequent identification of the isolated particles by a range of specialized analytical techniques, including FTIR microscopy, SEM-EDX and Raman.
Principle
Raman microspectroscopy is a form of vibrational spectroscopy that harnesses the inelastic scattering of light, whereby the difference in wavelength between excitation and emission upon interaction with the sample is determined by molecular vibration.
Applications
Biopharmaceutica
Procedure
1. Sample preparation.
2. Set up the instrument and observe the sample according to the instructions of Raman microscope.
3. Data analysis.
2. Set up the instrument and observe the sample according to the instructions of Raman microscope.
3. Data analysis.
Materials
• Sample: Particle-based APIs (e.g., VLPs, liposomes, polymeric nanoparticles), Virus Particle, Protein Formulations
• Equipment: Raman Microscopy
• Equipment: Raman Microscopy
Notes
• Advantage: Can provide chemical identity of particles and complex particles, can be automated to provide particle distributions, images and morphology, no interference with water can perform in solution phase.
• Disadvantage: Fluorescence can interfere with signals. Small volume for analysis (~70 µL).
• Sample Size Range: 2 µm – mm.
• Disadvantage: Fluorescence can interfere with signals. Small volume for analysis (~70 µL).
• Sample Size Range: 2 µm – mm.
Other recommended products
-
Analysis of Particle Identification by Fourier Transform Infrared (FTIR) Microscopy (CAT#: STEM-B-0366-CJ)
-
Analysis of Particle Identification by SEM-EDS (CAT#: STEM-B-0368-CJ)
Wide range of applications
For various laboratory needs
Expert support
Professional service team
Fast delivery
Global shipping