Analysis of NSD3 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1879-LGZ)

Name: Analysis of NSD3 Gene (Mutation) by RT-qPCR
SKU: STEM-MT-1879-LGZ
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Introduction

Official Full Name: nuclear receptor binding SET domain protein 3
Also known as: KMT3F; KMT3G; WHISTLE; WHSC1L1; pp14328
This gene is related to the Wolf-Hirschhorn syndrome candidate-1 gene and encodes a protein with PWWP (proline-tryptophan-tryptophan-proline) domains. This protein methylates histone H3 at lysine residues 4 and 27, which represses gene transcription. Two alternatively spliced variants have been described.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.