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Analysis of Nesfatin-1 (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0455-LGZ)

Introduction

Nesfatin 1 (NES1) is a neuropeptide produced in the mammalian hypothalamus. It is involved in regulating hunger and fat storage. An increase in nesfatin-1 in the hypothalamus contributes to a reduction in hunger, "fullness," and, potentially, loss of body fat and weight. Nesfatin-1 is a polypeptide encoded in the N-terminal region of the protein precursor, Nucleobindin2 (NUCB2).

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Principle Applications Procedure Materials

Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Metabolic

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum
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