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Analysis of Myosin BP (cardiac) (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0448-LGZ)

Introduction

Myosin is a mechanical enzyme that interacts with actin filaments and hydrolyzes ATP to generate motion and force. This allows myosin to push the slide of actin filaments, create tension on the actin filaments, and walk along those filaments. Thus, myosin can regulate the structure and dynamics of the actin cytoskeleton and influence the localization and trafficking of cellular components. Different myosins are grouped according to their motor domains.




Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Cardiovascular

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum