Analysis of membrane proteins by Circular dichroism (CD) (CAT#: STEM-MB-0598-WXH)

Introduction

Membrane proteins play essential roles in a wide variety of physiological functions, such as ionic regulation, molecular recognition, energy transduction and cell adhesion. It has been estimated that approximately 30% of all open reading frames in the human genome are membrane proteins and that they represent more than 60% of current drug targets. CD spectroscopy has special relevance for the study of membrane proteins, which are difficult to crystallise and largely ignored in structural genomics projects.

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Principle Applications Procedure Materials Notes Related Products

Principle

Circular dichroism (CD) is a spectroscopy technique that measures the absorption difference between left and right circularly polarized light. By symmetry, this asymmetric absorption can only occur for asymmetric molecules, meaning chiral molecules.

Applications

Circular dichroism (CD) spectroscopy is a powerful technique that is sensitive to the chirality (handedness) of molecules. It can be used to study absolute stereochemistry, enantiomeric composition, racemization, enantiomeric differentiation, and molecular interactions and conformation.

Procedure

1. Sample preparation
2. Measurement by CD instrument
3. Data analysis

Materials

Circular dichroism (CD) spectrophotometer

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