Analysis of Melamine by ELISA (CAT#: STEM-MB-2245-LGZ)

Name: Analysis of Melamine by ELISA
SKU: STEM-MB-2245-LGZ
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Introduction

Melamine is an important nitrogen heterocyclic organic chemical raw material. It is white crystalline powder. Chronic or repeated intake of melamine in large quantities may affect kidneys and bladder, which may lead to stone production. At present, melamine detection methods include: gas chromatography mass spectrometry (GC-MS), liquid chromatography tandem mass spectrometry (LC-MS-MS), liquid chromatography and so on. But the operation is complicated and the cost is high. Compared with instrumental analysis, ELISA has the characteristics of rapid, simple, accurate and sensitive. Can minimize the operation error and work intensity.

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Principle Applications Procedure Materials

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

For food safety.

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.