Analysis of MARK1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0402-LGZ)

Name: Analysis of MARK1 Gene (Mutation) by RT-qPCR
SKU: STEM-MT-0402-LGZ
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Introduction

Official Full Name: microtubule affinity regulating kinase 1
Also known as: MARK; Par1c; Par-1c
Enables several functions, including ATP binding activity; phospholipid binding activity; and protein kinase activity. Involved in intracellular signal transduction and protein phosphorylation. Located in cytoplasm; dendrite; and plasma membrane.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.