Analysis of LYVE1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2274-LGZ)

Name: Analysis of LYVE1 Gene (Mutation) by RT-qPCR
SKU: STEM-MT-2274-LGZ
Rating: 5 (1 reviews)

Introduction

Official Full Name: lymphatic vessel endothelial hyaluronan receptor 1
Also known as: HAR; XLKD1; LYVE-1; CRSBP-1
This gene encodes a type I integral membrane glycoprotein. The encoded protein acts as a receptor and binds to both soluble and immobilized hyaluronan. This protein may function in lymphatic hyaluronan transport and have a role in tumor metastasis.

Buy Now

Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.