Analysis of KIT D816V Mutation by RT-qPCR (CAT#: STEM-MT-0006-LGZ)

Name: Analysis of KIT D816V Mutation by RT-qPCR
SKU: STEM-MT-0006-LGZ
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Introduction

Mastocytosis is a heterogeneous clonal disorder with varying clinical features and prognosis, characterized by the proliferation and accumulation of mast cells (mast cells) in different tissues, mainly in the skin and bone marrow. Cutaneous mastocytosis (CM) is the most common form in children, while adults are almost always affected by a systemic disease (SM) characterized by involvement of at least one or more extracutaneous (EC) organs, with or without skin lesions. Most SM patients have a somatic "autoactivating" point mutation at codon 816 of the KIT receptor gene.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Mastocytosis

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.