Analysis of KCNN3 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1078-LGZ)

Name: Analysis of KCNN3 Gene (Mutation) by RT-qPCR
SKU: STEM-MT-1078-LGZ
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Introduction

Official Full Name: potassium calcium-activated channel subfamily N member 3
Also known as: SK3; ZLS3; hSK3; SKCA3; KCa2.3
The action potential in vertebrate neurons is followed by an afterhyperpolarization (AHP) that lasts for several seconds and can have profound effects on the neuronal firing pattern. Each component of AHP is kinetically distinct and mediated by distinct calcium-activated potassium channels. This gene belongs to the KCNN family of potassium ion channels. It encodes an integral membrane protein that forms a voltage-independent calcium-activated channel that is thought to regulate neuronal excitability by promoting the slow component of synaptic AHP. The gene contains two CAG repeat regions in the coding sequence. It was thought that expansion of one or both of these repeats might lead to increased susceptibility to schizophrenia or bipolar disorder, but research suggests that may not be the case. Alternative splice transcript variants of this gene have been found to encode distinct isoforms.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.