Analysis of KCNK2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1079-LGZ)

Name: Analysis of KCNK2 Gene (Mutation) by RT-qPCR
SKU: STEM-MT-1079-LGZ
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Introduction

Official Full Name: potassium two pore domain channel subfamily K member 2
Also known as: TREK; TPKC1; TREK1; K2p2.1; TREK-1; hTREK-1c; hTREK-1e
This gene encodes one of the members of the two-pore-domain background potassium channel protein family. This type of potassium channel is formed by two homodimers that create a channel that leaks potassium out of the cell to control resting membrane potential. The channel can be opened, however, by certain anesthetics, membrane stretching, intracellular acidosis, and heat. Three transcript variants encoding different isoforms have been found for this gene.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.