Analysis of ILDR2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1066-LGZ)

Name: Analysis of ILDR2 Gene (Mutation) by RT-qPCR
SKU: STEM-MT-1066-LGZ
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Introduction

Official Full Name: immunoglobulin like domain containing receptor 2
Also known as: C1orf32; dJ782G3.1
Predicted to act upstream of or within several processes, including homeostasis of number of cells within a tissue; insulin secretion; and response to glucose. Predicted to be located in endoplasmic reticulum membrane. Predicted to be integral component of membrane.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.