Analysis of IL-6R (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0358-LGZ)

Introduction

Interleukin-6 receptor (IL-6R) is the receptor for IL-6 and belongs to the type I cytokine receptor family, subfamily 3. IL-6 is a potent pleiotropic cytokine that regulates cell growth and differentiation and plays an important role in the immune response. IL-6R is a protein complex consisting of this protein and the interleukin 6 signal transducer (IL6ST/GP130/IL6β), a receptor subunit also shared by many other cytokines. Dysregulated production of IL-6 and this receptor has been implicated in the pathogenesis of many diseases such as multiple myeloma, autoimmune diseases and prostate cancer.

STEMart technology detail



Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Immunology/Inflammation

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum
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