Analysis of IL-34 (Human) by ELISA (CAT#: STEM-MB-0982-LGZ)

Name: Analysis of IL-34 (Human) by ELISA
SKU: STEM-MB-0982-LGZ
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Introduction

Interleukin 34 (IL-34) is a protein that is part of a group of cytokines known as interleukins. It was originally discovered in humans, and orthologs of interleukin-34 in chimpanzee, mouse, rat, and chicken were also discovered through large-scale screens of secreted proteins. The protein consists of 241 amino acids, has a mass of 39 kilodaltons, and forms homodimers. IL-34 increases the growth or survival of immune cells called monocytes, which it activates by binding to the colony-stimulating factor 1 receptor.

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Principle Applications Procedure Materials

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Metabolic, Immunology/Inflammation

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.