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Analysis of IL-1β (Non-human primate) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0327-LGZ)

Introduction

Interleukin-1 (IL-1) is a cytokine in the chemokine family. A total of 38 interleukins have been found, named IL-1---IL38. They have complex functions. Interleukin-1 is also known as lymphocyte stimulating factor. Mainly produced by activated monocyte-macrophages. The main forms are IL-1α and IL-1β, which can synergistically stimulate the activation of APC and T cells, promote the proliferation of B cells and secrete antibodies, induce the synthesis of proteins in the acute phase of the liver, and cause fever and cachexia.




Principle

Detection Technique: MSD Hypersensitive Multi-Factor Electrochemiluminescence
Capture Antibody: Mouse Monoclonal
Detection Antibody: Goat Polyclonal
Recombinant Standards: Expressed in E. coli

Applications

Cytokines & Chemokines, Immunology/Inflammation

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Serum, Plasma, Urine, Cell Culture Supernatant