Analysis of ICOS-L/B7-H2 (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0264-LGZ)
Introduction
ICOS is a homodimeric protein expressed on activated T cells and only at low levels on resting naive T cells. ICOS specifically binds to its ligand (ICOS-L), also known as B7-related protein-1 (B7RP-1), which is constitutively expressed on B cells.
MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.
Applications
Immunology/Inflammation
Procedure
1. Coat the capture antibody on the well plate. 2. Add samples/calibrators. 3. Add detection antibody. 4. Read the plate and analyze the data.