Analysis of I-309 (Human) by ELISA (CAT#: STEM-MB-0897-LGZ)

Name: Analysis of I-309 (Human) by ELISA
SKU: STEM-MB-0897-LGZ
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Introduction

Human I-309/CCL1 was originally identified by subtractive hybridization as a transcript present in γ/δ T cell lines but not in EBV-transformed B cells. Human CCL1 is considered to be a homologue of mouse TCA3. Although the two proteins share only about 42% of their amino acid sequence, both chemokines contain an extra pair of cysteine residues that are not found in most other chemokines. Human CCL1 and mouse TCA3 also showed significant sequence homology in the 5' flanking region of their genes.

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Principle Applications Procedure Materials

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Cytokines & Chemokines, Immunology/Inflammation

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.