Analysis of human subtelomeres by Fluorescence in situ hybridisation (FISH) (CAT#: STEM-MB-1218-WXH)

Name: Analysis of human subtelomeres by Fluorescence in situ hybridisation (FISH)
SKU: STEM-MB-1218-WXH
Rating: 5 (1 reviews)

Introduction

Subtelomeres are regions immediately adjacent to the telomeric repeats. They consist of repetitions of heterogeneous sequences, and have, therefore, been difficult to map and sequence. Cryptic subtelomeric chromosome rearrangements play an important role in the aetiology of mental retardation, congenital anomalies, miscarriages and neoplasia.

Buy Now

Principle Applications Procedure Materials

Principle

FISH uses fluorescent probes with complementary base sequences to locate the presence or absence of specific portions of DNA on chromosomes. The probe and target DNA must be denatured with heat or chemicals to break hydrogen bonds in the DNA and to allow hybridisation to occur once the two samples are mixed. The fluorescent probes form new hydrogen bonds with their complementary base pairs on the DNA, and these can then be detected via microscopy.

Applications

Detect and localize the presence or absence of specific DNA sequences on chromosomes.
Detect and localize specific RNA targets (mRNA, lncRNA and miRNA) in cells, circulating tumor cells, and tissue samples.

Procedure

1. Sample preparation
2. Co-denaturation and hybridization
3. Probe detection
4. Wash off of unbound probe
5. Analysis by flow cytometer/fluorescence microscopy