Analysis of HGF (Human) by ELISA (CAT#: STEM-MB-0891-LGZ)

Name: Analysis of HGF (Human) by ELISA
SKU: STEM-MB-0891-LGZ
Rating: 5 (1 reviews)

Introduction

Hepatocyte growth factor (HGF) was initially found to have a mitogenic effect on cultured hepatocytes. Later, HGF was successively shown to have mitotic effects on endothelial cells, epithelial cells, melanocytes and keratinocytes. Recently, HGF has also been found to promote migration of endothelial cells and epithelial cells. The natural HGF secreted by cells is an inactive single chain, which is cleaved into active enzyme by serine protease in serum. Mature HGF consists of a dimer of two single chains linked by a disulfide bond.

Buy Now

Principle Applications Procedure Materials

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Cardiac Biomarkers, Cardiac Injury, Cardiovascular, Toxicology

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.