Analysis of Hemopexin (Human) by ELISA (CAT#: STEM-MB-0889-LGZ)

Name: Analysis of Hemopexin (Human) by ELISA
SKU: STEM-MB-0889-LGZ
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Introduction

Hemopexin (Hpx) is a single-chain polypeptide, an acute phase response protein synthesized by the liver. It has a specific binding ability to free heme. When extensive hemolysis occurs, plasma haptoglobin is depleted, and free hemoglobin in the circulation can be degraded into globin and heme. Heme is insoluble in water and can be combined with Hpx to form a complex and transported to the liver.

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Principle Applications Procedure Materials

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Cardiovascular

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.