Analysis of HCC-4/CCL16 (Human) by ELISA (CAT#: STEM-MB-0879-LGZ)

Name: Analysis of HCC-4/CCL16 (Human) by ELISA
SKU: STEM-MB-0879-LGZ
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Introduction

CCL16, also known as CC chemokine 4, liver expression chemokine (LEC) and monotactin-1 (MTN-1), is a small cytokine in the CC chemokine family and a group of chemokines located on human chromosome 17. CCL16 is also a ligand for the histamine H4 receptor. CCL16 chemotaxis monocytes and lymphocytes, but not neutrophils. Recent studies have shown that CCL16 increases tumor rejection, macrophage antigen presentation, and angiogenic activity of vascular endothelial cells. On the other hand, CCL16 may also enhance the anticancer effects of cytotoxic T cells and dendritic cell (DC) lymphocytes.

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Principle Applications Procedure Materials

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Cytokines & Chemokines, Immunology/Inflammation

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.