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Analysis of Granulysin (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0229-LGZ)
Introduction
Granulysin (GNLY) is a cytotoxic protein present in the cytoplasmic granules of human cytotoxic T lymphocytes and natural killer cells. Amino acid sequence analysis showed that it is homologous to NK lysin and amoebalysin (antimicrobial protein), and is a member of saposin-likeprotein, SAPLIP family.
Principle
MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.
Applications
Oncology
Procedure
1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.
Materials
• Sample Type: Plasma, Serum
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