Analysis of Ghrelin (active) (Rat) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0209-LGZ)

Introduction

GHRL encodes the ghrelin-obesstatin preproprotein, which produces ghrelin and obestatin. Ghrelin is a ligand of growth hormone secretagogue receptor type 1, which is involved in regulating the release of growth hormone. Ghrelin has an appetite-stimulating effect, induces obesity, and stimulates gastric acid secretion.

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Principle

Detection Technique: MSD Hypersensitive Multi-Factor Electrochemiluminescence
Capture Antibody: Mouse Monoclonal
Detection Antibody: Rabbit polyclonal

Applications

Metabolic

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Cell Culture Media, EDTA Plasma, P800 Plasma, Serum
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