Analysis of GGCX Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1680-LGZ)

Name: Analysis of GGCX Gene (Mutation) by RT-qPCR
SKU: STEM-MT-1680-LGZ
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Introduction

Official Full Name: gamma-glutamyl carboxylase
Also known as: VKCFD1
This gene encodes an integral membrane protein of the crude endoplasmic reticulum that carboxylates glutamate residues of vitamin K-dependent proteins to gamma-carboxyglutamate, a modification necessary for their activity. The vitamin K-dependent protein substrate has an enzyme-binding propeptide with carbon dioxide, carbon dioxide, and reduced vitamin K as cosubstrates. Vitamin K-dependent proteins affect many physiological processes, including blood coagulation, prevention of vascular calcification, and inflammation. Allelic variants in this gene have been associated with multiple coagulation factor deficiencies associated with pseudoxanthoma elasticum. Alternative splicing results in multiple transcript variants.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.