Analysis of Genomic Variations Among Geographic Isolates of White Spot Syndrome Virus by Southern Blot Technology (CAT#: STEM-MHT-0122-LGZ)

Name: Analysis of Genomic Variations Among Geographic Isolates of White Spot Syndrome Virus by Southern Blot Technology
SKU: STEM-MHT-0122-LGZ
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Introduction

White spot syndrome virus (WSSV) is widely distributed in most Asian countries where prawns are farmed, and in some parts of the United States. WSSV from China, India, Thailand, prawns from Texas and South Carolina, and crayfish from the National Zoo in Washington, USA were analyzed by Southern blot.

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Principle Applications Procedure Materials

Principle

Under certain conditions, two single strands of nucleic acid with certain homology can be specifically hybridized to form double strands according to the principle of base complementarity. Generally, DNA molecules to be detected are digested with restriction enzymes, separated by agar-gel electrophoresis, denatured and transferred to nitrocellulocellulose film or nylon film or other solid phase support according to their position in the gel, fixed and then reacted with DNA probes labeled with isotopes or other markers. This is followed by autoradiography or an enzyme reaction to detect the amount of specific DNA molecules. If the object to be tested contains a sequence that is complementary to the probe, the two are combined by the principle of base complementarity, and the free probe is washed and detected by self-development or other suitable techniques, thus revealing the fragment to be tested and its relative size.

Applications

DNA Analysis

Procedure

1. Sample Processing
2. DNA Extraction and Digestion
3. Gel Electrophoresis
4. Gel Pretreatment
5. Transfer membrane
6. Probe Labeling
7. Prehybridization (blocking)
8. Southern hybridization
9. Membrane washing
10. Autoradiographic Assay
11. Results Analysis