Analysis of FGF-21 (Mouse) by ELISA (CAT#: STEM-MB-0813-LGZ)

Introduction

Fibroblast growth factor 21 (FGF-21) is expressed in many tissues, including liver, brown adipose tissue, white adipose tissue, and pancreas, and in mice, circulating FGF-21 originates from the liver. FGF-21 promotes glucose uptake by fat cells but has no effect on other types of cells. Serum FGF-21 is significantly elevated in patients with type II diabetes (T2DM), suggesting its role in the pathogenesis of T2DM. Elevated FGF-21 is also associated with liver fat content in nonalcoholic fatty liver disease and is positively correlated with BMI in humans, suggesting that obesity is a consequence of tolerance to FGF-21.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Metabolic

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or other biological fluids

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