Analysis of Eotaxin-2 (Human) by ELISA (CAT#: STEM-MB-0786-LGZ)

Name: Analysis of Eotaxin-2 (Human) by ELISA
SKU: STEM-MB-0786-LGZ
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Introduction

CCL24, also known as eosinophilic chemotactic protein 2 (eotaxin-2), bone marrow progenitor inhibitory factor 2 (MPIF-2), a small cytokine of the CC chemokine family, is located on chromosome 7 in the human genome. CCL24 showed strong chemotactic activity against resting T lymphocytes and eosinophils, and low chemotactic activity against neutrophils, but no chemotactic activity against monocytes and activated lymphocytes. By binding to its only receptor, CCR3, which is found primarily on eosinophils, but also on basophils, monocytes, Th2 lymphocytes, epithelial cells, and airway smooth muscle. CCL24 mediates atopic diseases, parasitic infections and systemic diseases. It also promotes cell transport and regulates inflammatory and fibrotic activity.

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Principle Applications Procedure Materials

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Cytokines & Chemokines, Immunology/Inflammation

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.