Analysis of ENTREP3 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0926-LGZ)

Name: Analysis of ENTREP3 Gene (Mutation) by RT-qPCR
SKU: STEM-MT-0926-LGZ
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Introduction

Official Full Name: endosomal transmembrane epsin interactor 3
Also known as: COTE1; C1orf2; FAM189B
This gene is located near the lysosomal glycosylceramidase gene; a deficiency of this enzyme is associated with Gaucher disease. The encoded protein has been identified as a potential binding partner of WW domain proteins involved in apoptosis and tumor suppression. Alternative splicing results in multiple transcript variants.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.